Journal: Scientific Reports

Article Title: Macrophages recruited by implanted fibrin gels promote regeneration of injured lymphatic vessels

doi: 10.1038/s41598-026-39167-2

Figure Lengend Snippet: Fibrin gel implantation leads to increased tissue CCL5 after lymphatic vessel injury and loss of CCL5 inhibits the recovery of lymphatic filling proximal to the injury site. ( a ) A comparative analysis of the levels of cytokines and growth factors after gel implantation demonstrating the upregulation of CCL5 and MMP9 ( n = 3 mice pooled in each group). Data generated by Proteome Profiler Cytokine Array (R&D systems, Minneapolis, MN) according to the manufacturer’s protocol. The values indicate fold changes in different groups relative to the values in the sham group. ( b ) Mice lacking CCL5 (CCL5-/-) cytokine are less likely than control mice to regain filling of vessels proximal to the injury site (proximal filling) subsequent to gel implantation 4-week post-injury ( p = 0.0498, two-sided Fisher’s exact test) while deficiency of CX3CR1 and CCR2 are not associated with the success/failure of proximal filling recovery following gel implantation ( p = 0.5105, two-sided Fisher’s exact test). ( c ) A comparison of lymphatic pumping frequencies via NIR technique proximal and distal to the injury site among CCL5-KO, CX3CR1(GFP/GFP), CCR2(-/-), and double-deficient CX3CR1(GFP/GFP)-CCR2(RFP/RFP) mice following gel implantation. To test the association between categorical variables (success/ failure of proximal filling after gel implantation), the two-sided Fisher’s exact test was used ( p < 0.05). One-way ANOVA analysis with the Tukey post hoc test was used to test the significant difference between different groups after gel implantation (* p < 0.05, ** p < 0.01, *** p < 0.001). All data were presented as mean (± s.e.m.).

Article Snippet: PROX1-eGFP (kind gift of Taija Mäkinen, Uppsala University, and Young-Kwon Hong, University of Southern California), CX3CR1 GFP/+ and CX3CR1 GFP/+ CCR2 RFP/+ reporter mice (C57BL/6, JAX), double mutant CX3CR1 GFP/RFP CCR2 RFP/RFP mice (C57BL/6, JAX), and CCL5-KO mice (C57BL/6, JAX) were housed in MGH Center for Comparative Medicine facilities.

Techniques: Generated, Control, Comparison

Journal: bioRxiv

Article Title: FYCO1 improves postischemic cardiac remodeling via enhanced autophagic flux and attenuation of proinflammatory signaling

doi: 10.64898/2026.03.17.712297

Figure Lengend Snippet: (a) Schematic representation of in vivo -RFP-EGFP-LC3-construct. (b,d) Illustrative confocal images showing colocalization of RFP (red) and EGFP (green) of RFP-EGFP-LC3 tandem fluorophore in immunohistochemistry demonstrating autophagosome (RFP+ EGFP+; yellow) and autolysosome (RFP+ EGFP-; red) presence in heart sections of RGFP and FYCO1-TGxRGFP mice. The graphs represent the statistical quantification of colocalization analysis of red and green fluorescence in confocal microscopy images 3 days (c) and 30 days (e) post MI (n=3-9, 6 images analyzed per section).

Article Snippet: In vivo, autophagic flux was assessed using RFP-EGFP-LC3 transgenic reporter mice (C57BL/6-Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/J, strain# 027139, The Jackson Laboratory, Bar Harbor, ME USA).

Techniques: In Vivo, Construct, Immunohistochemistry, Fluorescence, Confocal Microscopy

Journal: bioRxiv

Article Title: FYCO1 improves postischemic cardiac remodeling via enhanced autophagic flux and attenuation of proinflammatory signaling

doi: 10.64898/2026.03.17.712297

Figure Lengend Snippet: (a) Schematic representation of in vivo -RFP-EGFP-LC3-construct. (b,d) Illustrative confocal images showing colocalization of RFP (red) and EGFP (green) of RFP-EGFP-LC3 tandem fluorophore in immunohistochemistry demonstrating autophagosome (RFP+ EGFP+; yellow) and autolysosome (RFP+ EGFP-; red) presence in heart sections of RGFP and FYCO1-TGxRGFP mice. The graphs represent the statistical quantification of colocalization analysis of red and green fluorescence in confocal microscopy images 3 days (c) and 30 days (e) post MI (n=3-9, 6 images analyzed per section).

Article Snippet: Double-transgenic mice cardiomyocyte-specific FYCO1-overexpressing alongside a RFP-EGFP-LC3 reporter (C57BL/6-Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/J, strain# 027139, The Jackson Laboratory, Bar Harbor, ME USA), as well as their RFP-EGFP-LC3 reporter littermates (8-12 weeks old) were bred and housed in a temperature- and humidity-controlled facility on a 12-h light/dark cycle with free access to food and water.

Techniques: In Vivo, Construct, Immunohistochemistry, Fluorescence, Confocal Microscopy

Journal: bioRxiv

Article Title: FYCO1 improves postischemic cardiac remodeling via enhanced autophagic flux and attenuation of proinflammatory signaling

doi: 10.64898/2026.03.17.712297

Figure Lengend Snippet: (a) Schematic representation of in vivo -RFP-EGFP-LC3-construct. (b,d) Illustrative confocal images showing colocalization of RFP (red) and EGFP (green) of RFP-EGFP-LC3 tandem fluorophore in immunohistochemistry demonstrating autophagosome (RFP+ EGFP+; yellow) and autolysosome (RFP+ EGFP-; red) presence in heart sections of RGFP and FYCO1-TGxRGFP mice. The graphs represent the statistical quantification of colocalization analysis of red and green fluorescence in confocal microscopy images 3 days (c) and 30 days (e) post MI (n=3-9, 6 images analyzed per section).

Article Snippet: Double-transgenic mice cardiomyocyte-specific FYCO1-overexpressing alongside a RFP-EGFP-LC3 reporter (C57BL/6-Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/J, strain# 027139, The Jackson Laboratory, Bar Harbor, ME USA), as well as their RFP-EGFP-LC3 reporter littermates (8-12 weeks old) were bred and housed in a temperature- and humidity-controlled facility on a 12-h light/dark cycle with free access to food and water.

Techniques: In Vivo, Construct, Immunohistochemistry, Fluorescence, Confocal Microscopy